Route:
409 Highpoint SC - Yarraville
- Choose your direction:
 - to Highpoint SC
 - to Yarraville
 
409 to Highpoint SC
No scheduled service for the 409 to Highpoint SC at this time.
- Yarraville Station/Birmingham St (Yarraville) 
 - Simpson St/Fehon St (Yarraville) 
 - Blackwood St/Fehon St (Yarraville) 
 - Francis St/Fehon St (Yarraville) 
 - Bunninyong St/Francis St (Yarraville) 
 - Ballarat St/Francis St (Yarraville) 
 - Stephen St/Francis St (Yarraville) 
 - Hyde St/Francis St (Yarraville) 
 - Kent St/Hyde St (Yarraville) 
 - 204 Hyde St (Yarraville) 
 - Lennox St/Hyde St (Yarraville) 
 - Somerville Rd/Hyde St (Yarraville) 
 - Princess St/Hyde St (Footscray) 
 - Lyons St/Hyde St (Footscray) 
 - Footscray Town Hall/Hyde St (Footscray) 
 - Irving St/Nicholson St (Footscray) 
 - Footscray Station/Irving St (Footscray) 
 - Donald St/Moore St (Footscray) 
 - Stirling St/Moore St (Footscray) 
 - Footscray City College/Farnsworth Ave (Footscray) 
 - Doug Mills Reserve/Farnsworth Ave (Footscray) 
 - 70 Cumberland Dr (Maribyrnong) 
 - Cormorant Pl/Cumberland Dr (Maribyrnong) 
 - Bracken Ave/Edgewater Bvd (Maribyrnong) 
 - Jacks Way/Edgewater Bvd (Maribyrnong) 
 - Edgewater Shopping Centre/Edgewater Bvd (Maribyrnong) 
 - Macedon St/Gordon St (Maribyrnong) 
 - Highpoint Shopping Centre/Aquatic Dr (Maribyrnong) 
 - Highpoint SC/Rosamond Rd (Maribyrnong) 
 
409 to Yarraville
No scheduled service for the 409 to Yarraville at this time.
- Highpoint SC/Rosamond Rd (Maribyrnong) 
 - Highpoint Shopping Centre/Aquatic Dr (Maribyrnong) 
 - Lyric St/Gordon St (Maribyrnong) 
 - Edgewater Shopping Centre/Edgewater Bvd (Maribyrnong) 
 - Jacks Way/Edgewater Bvd (Maribyrnong) 
 - Bracken Ave/Edgewater Bvd (Maribyrnong) 
 - Cormorant Pl/Cumberland Dr (Maribyrnong) 
 - 70 Cumberland Dr (Maribyrnong) 
 - Henry Turner South Reserve/Farnsworth Ave (Footscray) 
 - Footscray City College/Farnsworth Ave (Footscray) 
 - Victoria University/Ballarat Rd (Footscray) 
 - Lynch St/Moore St (Footscray) 
 - Donald St/Moore St (Footscray) 
 - Footscray Station/Irving St (Footscray) 
 - Victoria University (Nicholson)/Albert St (Footscray) 
 - Footscray City PS/Bristow St (Footscray) 
 - Lyons St/Hyde St (Footscray) 
 - Harris St/Hyde St (Footscray) 
 - Lennox St/Hyde St (Yarraville) 
 - Earsdon St/Hyde St (Yarraville) 
 - Little Hyde St/Hyde St (Yarraville) 
 - Hyde St/Francis St (Yarraville) 
 - Stephen St/Francis St (Yarraville) 
 - Hughes St/Francis St (Yarraville) 
 - Hance St/Fehon St (Yarraville) 
 - Simpson St/Fehon St (Yarraville) 
 - Yarraville Station/Birmingham St (Yarraville)